ProteinPilot™ is a proteomics software program used for analysis of peptide tandem mass spectrometry data and for relative quantification of proteins using the iTRAQ® technology and other modes of stable isotope labeling. Raw data acquired on a mass spectrometer such as Orbitrap-Velos is searched against protein sequence databases; the Paragon scoring algorithm provides confidence levels for protein hits, and the ProGroup algorithm tool (PMID: 17533153) groups related and homologous proteins.The tutorial will include an introduction to the software followed by iTRAQ® Data analysis workflow and FDR analysis overviews within Galaxy-P.
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